In order to quantitate protein in biological samples, these several methods, Bradford, Lowry, BCA, Bohlen, has limitation since the constituents of biological buffers sometimes interfere with those methods. Therefore, it is limiting their application. But don’t worry, there is still a method that could be used to estimate protein in biological samples. The method is …
Column liquid chromatography has been employed for the separation, purification, and detection of nucleic acids. Among chromatography methods provided, anion-exchange chromatography has been most commonly employed for the isolation and purification of not only oligonucleotides but also large double-stranded DNA.
Some polymerase chain reaction (PCR) applications such as gene detection or typing, require little purified DNA and may be performed with crude bacterial extract. Many methods have been described for this procedure and some of them are straightforward and consist simply boiling bacterial cells in water. At this chance, we want to share the extraction …
Although Lowry method is not good enough compared to Bradford and Bicinchoninic Acid method for protein quantitation, but it is still widely used due to its acceptability to estimate protein in almost all circumstances in which protein mixtures or crude extracts are involved. Lowry method is based on the conversion of Cu2+ to Cu+ under …
In order to measure DNA content you can use UV Spectrophotometer with the advantages are nondestructive and allows the sample to be recovered for further analysis or manipulation. Spectrophotometry uses the fact that there is a relationship between the absorption of ultraviolet light by DNA/RNA and its concentration in a sample. In this particular posting, …
In the previous post, it had already explained about how to extract plasmid DNA by using alkaline lysis method. In this particular post, it will be explained the other method to extract plasmid DNA instead of using alkaline lysis method. It is Rapid Boiling method, an alternative to alkaline lysis method that was developed by …
Plasmids can be isolated by a variety of methods many of which rely on the differential denaturation and reannealing of plasmid DNA compared to chromosomal DNA. One commonly used technique developed by Birnboim and Doly involves alkaline lysis. This method essentially relies on bacterial lysis by sodium hydroxide and sodium dodecyl sulfate (SDS), followed by …