Polymerase chain reaction (PCR) is a primer mediated enzymatic amplification of specifically cloned or genomic DNA sequences. PCR process was invented by Kary Mullis and it has been automated for routine use in laboratories worldwide. The main purpose of the PCR process is to amplify template DNA using thermostable DNA polymerase enzyme which catalyzes the …
In this post, I want to share with you about 5 tips for successful PCR reaction since we often fail in conducting the PCR reactions. I believe this PCR method is easy to be conducted by anyone, but the handiness of the person is very required. Here are those 5 tips:
You must have known that agarose gel electrophoresis is generally adequate for resolving nucleic acid fragments in the size range of 100 nucleotides to around 10-15 kb. But, for nucleic acid which its fragments below those range, it will be difficult to separate and hard to visualize because of diffusion within the gel matrix. These …