Comments on: RNA Extraction From Fresh Blood http://bioteachnology.com/rna/rna-extraction-from-fresh-blood Wed, 08 Dec 2010 15:48:34 +0000 hourly 1 http://wordpress.org/?v=3.3.1 By: aquaplasmid http://bioteachnology.com/rna/rna-extraction-from-fresh-blood/comment-page-1#comment-172 aquaplasmid Sat, 12 Jun 2010 03:11:23 +0000 http://bioteachnology.com/?p=54#comment-172 We have made RNA extraction from fresh or frozen blood much simpler by using AquaPreserve solution. The following Q&A cues show some of the features of extracting blood RNA with AquaPreserve. Collecting blood in ACD vacutainers and storing them at -80°C is quite convenient, but we just couldn't get any usable RNA out of the frozen blood samples. Can AquaPreserve extract intact RNA from existing frozen whole blood? Yes. We need to collect blood samples in the field, can we stabilize, store, and ship whole blood in AquaPreserve at ambient temperatures? Yes. In addition to RNA, can AquaPreserve extract DNA and proteins from fresh and frozen whole blood? Yes. Lysing RBCs and enriching WBCs before RNA extraction not only throw away cell-free DNA, RNA, and virus in the plasma, but also induce artificial RNA profile. Does AquaPreserve protocol require RBC lysis? No. Do I have to buy additional DNA, RNA, and protein extraction kits and equipment to purify blood DNA, RNA, and proteins? No. With AquaPreserve, you can extract total RNA not only from fresh whole blood for prospective studies but also from archived frozen whole blood, which is the most accessible and often the only available biospecimen from the past, for retrospective studies. AquaPreserve may open new opportunities for your research and discovery! Hope AquaPreserve could meet your need of extracting total RNA from whole blood. We have made RNA extraction from fresh or frozen blood much simpler by using AquaPreserve solution. The following Q&A cues show some of the features of extracting blood RNA with AquaPreserve.

Collecting blood in ACD vacutainers and storing them at -80°C is quite convenient, but we just couldn’t get any usable RNA out of the frozen blood samples. Can AquaPreserve extract intact RNA from existing frozen whole blood? Yes.

We need to collect blood samples in the field, can we stabilize, store, and ship whole blood in AquaPreserve at ambient temperatures? Yes.

In addition to RNA, can AquaPreserve extract DNA and proteins from fresh and frozen whole blood? Yes.

Lysing RBCs and enriching WBCs before RNA extraction not only throw away cell-free DNA, RNA, and virus in the plasma, but also induce artificial RNA profile. Does AquaPreserve protocol require RBC lysis? No.

Do I have to buy additional DNA, RNA, and protein extraction kits and equipment to purify blood DNA, RNA, and proteins? No.

With AquaPreserve, you can extract total RNA not only from fresh whole blood for prospective studies but also from archived frozen whole blood, which is the most accessible and often the only available biospecimen from the past, for retrospective studies. AquaPreserve may open new opportunities for your research and discovery!

Hope AquaPreserve could meet your need of extracting total RNA from whole blood.

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