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Some polymerase chain reaction (PCR) applications such as gene detection or typing, require little purified DNA and may be performed with crude bacterial extract. Many methods have been described for this procedure and some of them are straightforward and consist simply boiling bacterial cells in water. At this chance, we want to share the extraction …

In the previous post, it had already explained about how to extract plasmid DNA by using alkaline lysis method. In this particular post, it will be explained the other method to extract plasmid DNA instead of using alkaline lysis method. It is Rapid Boiling method, an alternative to alkaline lysis method that was developed by …

You must have known that agarose gel electrophoresis is generally adequate for resolving nucleic acid fragments in the size range of 100 nucleotides to around 10-15 kb. But, for nucleic acid which its fragments below those range, it will be difficult to separate and hard to visualize because of diffusion within the gel matrix. These …