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Some polymerase chain reaction (PCR) applications such as gene detection or typing, require little purified DNA and may be performed with crude bacterial extract. Many methods have been described for this procedure and some of them are straightforward and consist simply boiling bacterial cells in water. At this chance, we want to share the extraction …

In the previous post, it had already explained about how to extract plasmid DNA by using alkaline lysis method. In this particular post, it will be explained the other method to extract plasmid DNA instead of using alkaline lysis method. It is Rapid Boiling method, an alternative to alkaline lysis method that was developed by …