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Although Lowry method is not good enough compared to Bradford and Bicinchoninic Acid method for protein quantitation, but it is still widely used due to its acceptability to estimate protein in almost all circumstances in which protein mixtures or crude extracts are involved. Lowry method is based on the conversion of Cu2+ to Cu+ under …

The Bicinchoninic acid (BCA) assay first was described by Smith, et al. BCA assay is similar to Lowry assay since it also depends on the conversion of Cu(2+) to Cu(+) under alkaline conditions. The Cu(+) is then detected by reaction with BCA. The reaction results in the development of an intense purple color with an …