Column liquid chromatography has been employed for the separation, purification, and detection of nucleic acids. Among chromatography methods provided, anion-exchange chromatography has been most commonly employed for the isolation and purification of not only oligonucleotides but also large double-stranded DNA.
Read more »Some polymerase chain reaction (PCR) applications such as gene detection or typing, require little purified DNA and may be performed with crude bacterial extract. Many methods have been described for this procedure and some of them are straightforward and consist simply boiling bacterial cells in water. At this chance, we want to share the extraction …
Read more »Polymerase chain reaction (PCR) is a primer mediated enzymatic amplification of specifically cloned or genomic DNA sequences. PCR process was invented by Kary Mullis and it has been automated for routine use in laboratories worldwide. The main purpose of the PCR process is to amplify template DNA using thermostable DNA polymerase enzyme which catalyzes the …
Read more »In this post, I want to share with you about 5 tips for successful PCR reaction since we often fail in conducting the PCR reactions. I believe this PCR method is easy to be conducted by anyone, but the handiness of the person is very required. Here are those 5 tips:
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