Measuring the purity of mRNA can be done by measuring its absorbance at 260 nm. An absorbance of 1.0at 260 nm is equivalent is equivalent to 40 mg mRNA. An additional reading at 280 nm allows the A260:A280 ratio to be calculated. A ratio of 2.0 should be expected. The integrity of the mRNA can …
Read more »How to make RNA get linear and prevent its secondary structure? In order to linearize or denature RNA, it can be achieved by heating to 80-90o for 5 minutes, then cooling quickly on ice (taking care not to cause precipitation of the loading-buffer components) and immediately applying it. The step above can reduce the RNA …
Read more »RNA can be extracted from blood since whole blood contains nucleated white cells that constitute an easily accessible source. RNA extraction from blood will be more successful if the nucleated white cells are first isolated from the red cells since the red cells are a rich source of ribonucleases that are able to degrade RNA. …
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In order to measure DNA content you can use UV Spectrophotometer with the advantages are nondestructive and allows the sample to be recovered for further analysis or manipulation. Spectrophotometry uses the fact that there is a relationship between the absorption of ultraviolet light by DNA/RNA and its concentration in a sample. In this particular posting, …
Read more »After it is extracted from particular sources, nucleic acid must be stored either for archival purposes or before assay performance. The principle of nucleic acid storage is prevention of enzymatic or physical damage to the purified product. In order to overcome those problems we have three options of method to store our purified nucleic acid: …
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